forensic science, forensic pathology, silage pit, accidental death, drowning, carbon dioxide, hydrogen sulfide, infrared gasanalyzer

Fatalities due to gas intoxication are rare in routine forensic casework. The most common gas is carbon monoxide, with other gases (ammonia, methane, propane and butane, carbon dioxide, hydrogen sulfide) encountered only very rarely. In this report, we describe the accidental death of two maintenance workers who were found in the silage pit at a biogas plant. The autopsy revealed signs of asphyxia in both the deceased. Analysis of the gaseous mixtures in the tank using an infrared gas analyzer showed slightly elevated concentrations of carbon dioxide. Toxicological examination of the blood of both the deceased using gas chromatography with a flame ionization detector, spectrophotometry and liquid chromatography-mass spectrometry did not detect any toxicologically significant substance that would explain the sudden collapse. Both the autopsy and the toxicology analyses suggest CO2 intoxication as the most likely cause for the collapse of the two men, which then led to sudden asphyxia. © 2018 American Academy of Forensic Sciences.