liver regeneration; Sus scrofa; associating liver partition and portal vein ligation for staged hepatectomy; stem cells; transcriptome sequencing

This present study investigated the impact of theapplication of stem cells to liver regeneration following the firststage of associating liver partition and portal vein ligation forstaged hepatectomy (ALPPS). The experiment was conductedon a pig model (n=6, 3 that did not receive application ofstem cells, 3 that received application stem cells). Collectedsamples of liver (day 0 and 9 following surgery) were subjectedto complete transcriptome sequencing. In total, 39 differen-tially expressed genes were found in the group without theapplication of the stem cells (genes of unwanted processessuch as fibrosis and inflammation). In the group that didreceive application of stem cells, no significantly differentiallyexpressed genes were found, indicating a properly regeneratedliver remnant. The present study therefore demonstrated, to thebest of our knowledge for the first time, the positive effect ofstem cells application in the liver regeneration process duringALPPS procedure in the pig model