zeta potential; BSA; mathematical processing

Introduction:In this study was measured and processed zeta potential of Bovine Serum Albumin (BSA) in different conditions. The zeta potential was determined in dependence on changing pH, temperature and ionic strength. The results were evaluated using statistics and influence of these parameters was investigated.Methods:Samples of BSA with concentration of 0.044mM?L-1 were prepared. pH was adjusted using NaOH (0.25M) and HCl (0.25M, 0.025M) for reach the values of pH in range 2 -11. Temperature during the experiment varied from 5°C to 60°C with 5°C step. Two salts were used (KCl and CaCl2 with concentrations 10-2, 10-3 and 10-4 M?L-1) to achieve different ionic strength.The zeta potential of these samples was measured using Zetasizer NanoZS instrument. It was measured by Electrophoresis and Laser Doppler Velocimetry. Results and Discussion:Measured data were compiled and processed. The data are filtered based on Zeta Potential Range. The probability densities of variables are then calculated with non-parametric kernel method.The application calculates probability density of the temperature and pH. It also estimates probability mass functions of the ionic strengths of both salts. The application calculates also modus of all parameters. Use of this application can help in following research for example for adsorption of BSA. It can be used for the preparation of samples with required zeta potential without preparation of several samples and lengthy treatment. From the measurement and with use of this application, it was found that the most significant influence on zeta potential have change of pH and presence of CaCl2. For reliable results of application is necessary to measure several samples of the same parameters but it is difficult to realize from experimental aspect. So there have to be found the balance between ideal conditions for statistics and actual experimental possibilities.