voltammetry boron-doped diamond electrode drug research

Novel voltammetric method for the determination of bromazepam (BZ) on BDDE was developed. Commercial (boron concentration of 1000 ppm, 3 mm diameter, Windsor Scientific Ltd.) and four lab-made BDD sensors with different boron doping level (0- 8000 ppm, 740 ?m diameter) were used as working electrodes. Lab-made BDD sensor (1000 ppm) was chosen as the most suitable electrode for the purpose of the work primarily because of high current response, reproducibility, and low background current. The optimal medium Britton-Robinson buffer of pH 11 was found for the detection and quantification of BZ. The cyclic voltammogram of BZ exhibits one cathodic peak at -1.07 V in the optimal medium. The presence of reduction peak in cyclic voltammogram and the shift of potential with increasing scan rate point to irreversible reduction of azomethine functional group in the BZ structure. The calibration curves were measured in the concentration range from 10-7 to 10-4 mol/L using differential pulse voltammetry with the achieved limit of detection 5×10-7 mol/L. The proposed voltammetric method was subsequently applied to analysis of BZ in pharmaceutical preparation Lexaurin with recovery 95.59 ? 3.92 % and RSD 2.92 %.

Novel voltammetric method for the determination of bromazepam (BZ) on BDDE was developed. Commercial (boron concentration of 1000 ppm, 3 mm diameter, Windsor Scientific Ltd.) and four lab-made BDD sensors with different boron doping level (0- 8000 ppm, 740 ?m diameter) were used as working electrodes. Lab-made BDD sensor (1000 ppm) was chosen as the most suitable electrode for the purpose of the work primarily because of high current response, reproducibility, and low background current. The optimal medium Britton-Robinson buffer of pH 11 was found for the detection and quantification of BZ. The cyclic voltammogram of BZ exhibits one cathodic peak at -1.07 V in the optimal medium. The presence of reduction peak in cyclic voltammogram and the shift of potential with increasing scan rate point to irreversible reduction of azomethine functional group in the BZ structure. The calibration curves were measured in the concentration range from 10-7 to 10-4 mol/L using differential pulse voltammetry with the achieved limit of detection 5×10-7 mol/L. The proposed voltammetric method was subsequently applied to analysis of BZ in pharmaceutical preparation Lexaurin with recovery 95.59 ? 3.92 % and RSD 2.92 %.