cerium oxide, bovine serum albumin, isotherms, kinetic, thermodynamic

Adsorption of bovine serum albumin on cerium oxide was studied, the influence of various parameters was followed. Increasing of pH led to decrease of zeta potential and decrease of adsorption capacity of cerium oxide nanoparticles. The samples of nanoceria with positive zeta potential adsorbed more bovine serum albumin on the other hand, the samples with negative zeta potential showed little or no protein adsorption. The adsorption capacity of BSA on the ceria was found to increase with rise in temperature for all studied samples. Linear as well as nonlinear regression of experimental data confirmed that adsorption process can be described using Langmuir adsorption theory of monolayer coverage. Calculated thermodynamic parameters indicate that the adsorption of BSA on the ceria is endothermic (?Hads > 0) and non-spontaneous (?Gads > 0) process. The kinetics of adsorption fitted best to pseudo-second-order.