lutein, HPLC/MS, determination

utein belongs to carotenoids xanthophylls. This xanthophyll can play many roles in biological organisms. Probably the most important role is its preventive effect against cataracts and age related macular degeneration (AMD) [1]. Lutein is essential for human health, but their abundance in the human body is entirely dependent on the dietary intake [1, 2]. Due to its potential therapeutic function, the presence of lutein in human diets has become of considerable interest. Thus, many nutritional supplements are enriched with lutein. For studying of stability and other properties of lutein many complicated analytical methods have been developed [3]. These methods are long, complicated, expensive and with critical conditions for mass detection. For this purpose we proposed simple, no time-consuming, many times cheaper method with suitable conditions for mass detection.