molecular phylogeny; Diptera; Sciaroidea

The phylogeny of selected genera from four subfamilies of fungus gnats (Diptera: Mycetophilidae) is reconstructed based on the combined analysis of five mitochondrial (12S , 16S , COI , COII , cytB) and two nuclear (28S , ITS2 ) gene markers. Results of the different analyses all support Manotinae as a monophyletic group, with Leiinae as the sister group. Allactoneura DeMeijere is nested in the monophyletic and strongly supported clade of Leiinae. The tribe Metanepsiini is revealed as paraphyletic. The genera Docosia Winnertz, Ectrepesthoneura Enderlein, Novakia Strobl and Syntemna Winnertz were placed with a group of genera included traditionally in the Gnoristinae. The monophyly of Dziedzickia Johannsen and Phthinia Winnertz is not supported. The genera of Sciophilinae (excluding Paratinia Mik but including Eudicrana Loew) form a monophyletic group in the Bayesian model.