HPLC, benzodiazepines

Benzodiazepines are the most popular psychotropic drugs by doctors and drug addicts in the present. They are used mainly as sedatives, anxiolytics. The side effects are mainly fatigue and sleepiness. The aim of this work was to developed HPLC/UV method for determination of six benzodiazepines. The method was developed on the instrument Agilent 1200, which was equipped with a column Gemini 5? C18 110A. The developed method had following parameters: mobile phase A - 0.01M ammonium acetate + 0.1% (v/v) formic acid, mobile phase B - acetonitrile, column temperature - 20°C and spray - 20 ?l. The parameters of method were following: LOD - 0,1 ?g/l, LOQ - 0,2 ?g/l, correlation linearity coefficients higher than 0,985, deviation of reproducibility and repeatability better than 7% and deviation in precision and accuracy better than 7%. The obtained HPLC/UV method is suitable for introduction into practical usage in hospital laboratories.