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Publikační činnost

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Typ záznamu * :	článek v odborném periodiku (J)
Domácí pracoviště * :	Katedra biologie a ekologie (31700)
Název * :	Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus Orthotrichum Using a Small Throughput Pyrosequencing Machine
Citace :	Sawicki, J., Kwaśniewski, M., Szczecińska, M., Chwiałkowska, K., Milewicz, M. a Plášek, V. Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus Orthotrichum Using a Small Throughput Pyrosequencing Machine. INT J MOL SCI. 2012, roč. 13, s. 7586-7593. ISSN 1422-0067.

Podnázev :
Rok * :	2012
Obor * :	Botanika
Kód ISSN * :	1422-0067
Oficiální název periodika * :	INT J MOL SCI
Stát vydavatele periodika * :	Švýcarská konfederace
Svazek periodika * :	Neuveden
Číslo periodika v rámci svazku * :	6
Číslo článku :
Ročník :	13
Počet stran článku * :	8
Strana od * :	7586
Strana do * :	7593
Kód UT WoS :
EID :
Poddruh recenzovaného článku :	Článek v impaktovaném časopise (Jimp)

Klíčová slova anglicky * :	SSR markers; Orthotrichum; 454 pyrosequencing
Popis v původním jazyce * :	Here, we report the results of next-generation sequencing on the GS Junior system to identify a large number of microsatellites from the epiphytic moss Orthotrichum speciosum. Using a combination of a total (non-enrichment) genomic library and small-scale 454 pyrosequencing, we determined 5382 contigs whose length ranged from 103 to 5445 bp. In this dataset we identified 92 SSR (simple sequence repeats) motifs in 89 contigs. Forty-six of these had flanking regions suitable for primer design. We tested PCR amplification, reproducibility, and the level of polymorphism of 46 primer pairs for Orthotrichum speciosum using 40 individuals from two populations. As a result, the designed primers revealed 35 polymorphic loci with more than two alleles detected. This method is cost- and time-effective in comparison with traditional approaches involving cloning and sequencing.
Popis v anglickém jazyce * :	Here, we report the results of next-generation sequencing on the GS Junior system to identify a large number of microsatellites from the epiphytic moss Orthotrichum speciosum. Using a combination of a total (non-enrichment) genomic library and small-scale 454 pyrosequencing, we determined 5382 contigs whose length ranged from 103 to 5445 bp. In this dataset we identified 92 SSR (simple sequence repeats) motifs in 89 contigs. Forty-six of these had flanking regions suitable for primer design. We tested PCR amplification, reproducibility, and the level of polymorphism of 46 primer pairs for Orthotrichum speciosum using 40 individuals from two populations. As a result, the designed primers revealed 35 polymorphic loci with more than two alleles detected. This method is cost- and time-effective in comparison with traditional approaches involving cloning and sequencing.

Typ zdroje financování výsledku * :

Projekty CEP/Záměry CEZ

Seznam projektů :

ID Projektu	Název projektu

Seznam ohlasů :

Ohlas

R01:	RIV/61988987:17310/12:A13014QT

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