EMTREE drug terms: virus DNA EMTREE medical terms: adult; aged; article; CD8+ T lymphocyte; cellular immunity; clinical article; cohort analysis; controlled study; Cytomegalovirus; cytomegalovirus infection; female; graft recipient; human; kidney transplantation; longitudinal study; lymphocyte count; male; postoperative complication; postoperative infection; priority journal; prospective study; quantitative assay; risk assessment; sensitivity and specificity; virus load MeSH: Adult; Aged; Antibodies, Viral; CD8-Positive T-Lymphocytes; Cytomegalovirus; Cytomegalovirus Infections; Czech Republic; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunity, Cellular; Immunosuppressive Agents; Interferon-gamma; Kidney Transplantation; Longitudinal Studies; Male; Middle Aged; Predictive Value of Tests; Prospective Studies; Reproducibility of Results; Risk Assessment; Sensitivity and Specificity; Treatment Outcome; Viral Load; Virus Latency; Young Adult

Infection with cytomegalovirus (CMV) is a major cause of morbidity and mortality in immunosuppressed patients, including organ and bone marrow transplant recipients. The majority of CMV disease is caused by reactivation of alatent infection rather that by newly acquired virus. Many techniques have been currently available to aid in the diagnostics of CMV disease. In this report we performed a prospective evaluation of Quantiferon-CMV assay (Cellestis) to determine whether the test is predictive of CMV disease. CD8+ T-cell CMV-specific immunity was assessed in a longitudinal cohort of 14 kidney transplant recipients. According to our data, subjects with higher cellular immune response measured with Quantiferon test had a lower risk of manifestation of CMV infection than subjects with lower responses...