molecular diversity; polypores; Ganoderma resinaceum; intraspecific variability

Ganoderma resinaceum Boud. is commonly found in Mediterranean region, but rarely in Western, Central or Eastern Europe. It is a parasitic basidiomycetous fungus causing stem decay-especially in urban trees. Acollection of nine fungal specimens from Slovakia (Central Europe), morphologically identified as G. resinaceum, was recently studied on the basis of sequence data from the internal transcribed spacer (ITS) regions. Analyses showed that the collections clustered into two separate groups. In this study-for the first time-the sequences of other molecular markers, namely partial translation elongation factor (tef1-α) region and partial 25S large subunit ribosomal RNA gene (25S LSU rRNA), as well as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI–TOF MS) were obtained and used to evaluate the genetic variability of G. resinaceum. All these analyses confirm the existence of two previously unrecognized genotypes within the morphospecies.