ibuprofen, mass spectrometry

Everybody knows headache and most of us resolve them by pill containing ibuprofen. It gets into surface water due to imperfections in metabolism and in the wastewater treatment plants. This work presents HPLC/MS method for the determination of ibuprofen applicable in the analysis of surface water. The mobile phase, which had flow 200 ?l/min, was prepared from acetonitril, methanol and water. Column temperature was 20°C and inject was 100 ?l. Detection was performed using a mass spectrometer in negative ionization mode with these parameters: endplate offset- - 500 V, capillary voltage - + 2900 W, nebulizer gas pressure - 2 bar, drying gas flow rate - 10 l/min, drying gas temperature - 200°C. The method was developed on the HPLC/MS system Ultimate 3000/microTOF-QII. The developed method was tested according to principles of validation. Obtained results show that the obtained HPLC/MS method for ibuprofen determination is suitable for practical usage in the analysis of surface water.