Crayfish; Astacus leptodyctylus; intersex; Potamopyrgus antipodarum; sediment; dioxine; steroidogenesis

Waste coal toxicity evaluation is evaluated by means of the endocrine disruptive potency of sediment contaminated by waste coal using in-vitro bioassays an in vivo sediment test. The material was taken from the localities (Karviná, Northern Moravia) where intersex characters had been observed on crayfish Astacus leptodactylus. Dioxin-like activity of sediments and alteration of CYP11B2, enzyme involved in mammalian steroidogenesis are examined. Sediment biotest with mud snail Potamopyrgus antipodarum was performed.

Waste coal toxicity evaluation is evaluated by means of the endocrine disruptive potency of sediment contaminated by waste coal using in-vitro bioassays an in vivo sediment test. The material was taken from the localities (Karviná, Northern Moravia) where intersex characters had been observed on crayfish Astacus leptodactylus. Dioxin-like activity of sediments and alteration of CYP11B2, enzyme involved in mammalian steroidogenesis are examined. Sediment biotest with mud snail Potamopyrgus antipodarum was performed.